chloroplast gene meaning in Chinese
叶绿体基因
Examples
- Ii . molecular phylogeny in this paper , nucleotide sequences of the chloroplast gene trnl - f region was investigated first in the ferns , the pcr primers were chosen . in the test , 34 species in athyriaceae and 3 outgroup taxa were determined trril - f region sequences
介蔗属d尽口athyrium 、蛾眉蔗属lunathyrium 、假蹄盖蔽属athyriopsis 、单叶双盖蔗diplaziumsubsinuatum组成一支，在两种系统树中均得到100 %的支持率。 - In order to form a chloroplast transformation system of d . salina , we have conducted some studies including its sensitivity to antibiotics , the activity of promoter , cloning of the chloroplast genes and construction of transformation vectors , so far a pilot transformation system of the d . salina chloroplast has been completed . methods : the sensitivity of d . salina to seven antibiotics or herbicide used commonly in gene engineering was studied and the biological activity of atpa promoter from c . reinhardtii chloroplast was tested by using enhanced green fluorescent protein ( egfp ) as a reporter . primers were designed in the conservative encoding regions according to the chloroplast genomes from four algae which have close genetic relationship with d . salina , and the sequences of 16s rrna , chll and chln of d . salina chloroplast were cloned and sequenced , respectively
方法:根据杜氏盐藻的近缘藻类的叶绿体基因组序列资料，在基因编码区的高度保守区域设计引物，克隆了杜氏盐藻叶绿体165识na基因、咖l基因和ch n基因，并分别以165识na基因和chln基因序列为同源片段，以cat和bar基因为筛选标记基因构建了三套杜氏盐藻叶绿体转化载体: 2郑州大学2003年博士学位论文杜氏盐藻( d ~ iiellasalina )叶绿体转化研究pds165一eaf 、 ptn1269一bar和psp72一5一bar一3 ，用基因枪法转化杜氏盐藻，初步建立起杜氏盐藻叶绿体转化体系。